气候变化领域集成服务门户(CCPortal): Functional analysis of FLOWERING LOCUS T orthologs from spring orchid (Cymbidium goeringii Rchb. f.) that regulates the vegetative to reproductive transition

CCPortal

DOI	10.1016/j.plaphy.2012.06.011
	Functional analysis of FLOWERING LOCUS T orthologs from spring orchid (Cymbidium goeringii Rchb. f.) that regulates the vegetative to reproductive transition
	Xiang, Lin; Li, Xiaobai; Qin, Dehui; Guo, Fangqi; Wu, Chao; Miao, Lixiang; Sun, Chongbo
发表日期	2012
ISSN	0981-9428
起始页码	98
结束页码	105
卷号	58
英文摘要	The FLOWERING LOCUS T (FT) gene plays crucial roles in regulating the vegetative-to-reproductive phase transition. The FT-like gene of spring orchid (Cymbidium goeringii Rchb. f.), CgFT, was isolated and characterized. CgFT mRNA was detected in leaves, pseudobulb, and flowers. In flowers, CgFT was expressed more in young flower buds than in mature flowers, and was predominantly expressed in young ovary and sheath. Seasonal expression analysis in leaves of a three-year-old spring orchid showed that a large increase in transcription, which started on June 20 for CgFT. We propose that the increased transcription in the middle of June marks the beginning of flower induction in this species. The ectopic expression of CgFT in transgenic tobacco plants showed novel phenotypes by flowering earlier than wild-type plants. Further analysis of the flowering time-related genes indicated that the expression of LEAFY, APETALLA1, FRUITFULL and SEPALLATA1 were significantly upregulated in 35S::CgFT transgenic tobacco plants. These results indicated that CgFT is a putative FT homolog in spring orchid that regulates flower transition, similar to its homolog in Arabidopsis. This study provides the first information on the spring orchid floral gene to elucidate the regulation of the flowering transition in spring orchid. (C) 2012 Elsevier Masson SAS. All rights reserved.
英文关键词	Flower induction; FLOWERING LOCUS T (FT); Gene expression; Spring orchid; Tobacco
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
来源期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158625
作者单位	Sun, CB (corresponding author), Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China.
推荐引用方式 GB/T 7714	Xiang, Lin,Li, Xiaobai,Qin, Dehui,et al. Functional analysis of FLOWERING LOCUS T orthologs from spring orchid (Cymbidium goeringii Rchb. f.) that regulates the vegetative to reproductive transition[J],2012,58.
APA	Xiang, Lin.,Li, Xiaobai.,Qin, Dehui.,Guo, Fangqi.,Wu, Chao.,...&Sun, Chongbo.(2012).Functional analysis of FLOWERING LOCUS T orthologs from spring orchid (Cymbidium goeringii Rchb. f.) that regulates the vegetative to reproductive transition.PLANT PHYSIOLOGY AND BIOCHEMISTRY,58.
MLA	Xiang, Lin,et al."Functional analysis of FLOWERING LOCUS T orthologs from spring orchid (Cymbidium goeringii Rchb. f.) that regulates the vegetative to reproductive transition".PLANT PHYSIOLOGY AND BIOCHEMISTRY 58(2012).