气候变化领域集成服务门户(CCPortal): Susceptibility of mountain pine beetle (Dendroctonus ponderosae Hopkins) to gene silencing through RNAi provides potential as a novel management tool

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DOI	10.1016/j.foreco.2020.118322
	Susceptibility of mountain pine beetle (Dendroctonus ponderosae Hopkins) to gene silencing through RNAi provides potential as a novel management tool
	Kyre B.R.; Bentz B.J.; Rieske L.K.
发表日期	2020
ISSN	0378-1127
卷号	473
英文摘要	The mountain pine beetle (MPB), Dendroctonus ponderosae, is an eruptive endemic forest pest that is undergoing substantial range expansion in response to recent climatic changes, breaching geographic barriers, exploiting novel hosts, and affecting millions of hectares of conifer forests in western North America. Current management approaches have been unable to keep pace with MPB population outbreaks, and novel and aggressive management responses are required as MPB's range expansion progresses. Gene silencing through RNA interference (RNAi) is an emerging pest management approach that is being developed for agricultural pests, and has also been shown to be effective against some xylophagous forest pests, including the southern pine beetle (SPB), D. frontalis. When essential genes are targeted, RNAi can cause rapid insect mortality; here we focus on evaluating its effectiveness in MPB. We identified reference genes for quantitative real-time PCR (qPCR) and validated RNAi responses in MPB by analyzing gene expression and beetle survival. Using an adult bioassay that combined oral ingestion and dermal absorption of dsRNAs targeting three genes (hsp, iap, and shi), we measure gene expression and demonstrate silencing, as well as insect mortality, following dsRNA exposure. All three genes were silenced and all treatment beetles died within 7 d. This validates reference genes for expression analyses and demonstrates that MPB, similar to the congeneric SPB, has a highly sensitive RNAi response. Additionally, we document sex-specific differences in gene expression for one of the three target genes, hsp; any differences in gene expression and subsequent mortality based on sex must be considered as this technology progresses as a pest management tool. RNAi causes rapid insect mortality when essential genes are targeted, is highly specific to the target pest, and has no environmental contamination risks, making it an attractive approach for further development in forest pest suppression. © 2020 The Author(s)
英文关键词	Climate change; Integrated pest management; RNA interference; Tree mortality
语种	英语
scopus关键词	Agricultural robots; Expansion; Gene expression; Genetic engineering; Polymerase chain reaction; Dendroctonus ponderosae; Environmental contamination; Expression analysis; Mountain pine beetle; Quantitative real time PCR; Sex-specific differences; Southern pine beetle; Technology progress; Forestry; beetle; climate change; coniferous forest; crop pest; endemic species; gene expression; pest species; range expansion; RNA; Expansion; Forestry; Genes; Genetic Engineering; Insects; Management; Mortality; Pinus Mugo; North America; Coleoptera; Coniferophyta; Dendroctonus frontalis; Dendroctonus ponderosae; Hexapoda; Pinus mugo
来源期刊	Forest Ecology and Management
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/155143
作者单位	Department of Entomology, University of Kentucky S225 Ag North, Lexington, KY 40546-0091, United States; USDA Forest Service Rocky Mountain Research Station, Logan, UT 84321, United States
推荐引用方式 GB/T 7714	Kyre B.R.,Bentz B.J.,Rieske L.K.. Susceptibility of mountain pine beetle (Dendroctonus ponderosae Hopkins) to gene silencing through RNAi provides potential as a novel management tool[J],2020,473.
APA	Kyre B.R.,Bentz B.J.,&Rieske L.K..(2020).Susceptibility of mountain pine beetle (Dendroctonus ponderosae Hopkins) to gene silencing through RNAi provides potential as a novel management tool.Forest Ecology and Management,473.
MLA	Kyre B.R.,et al."Susceptibility of mountain pine beetle (Dendroctonus ponderosae Hopkins) to gene silencing through RNAi provides potential as a novel management tool".Forest Ecology and Management 473(2020).