气候变化领域集成服务门户(CCPortal): Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics

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DOI	10.1088/1748-9326/ab7131
	Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics
	Zhang X.; Li J.; Li D.; Zhang H.; Hu H.
发表日期	2020
ISSN	17489318
卷号	15 期号:3
英文摘要	Nanoparticles (NPs) and antibiotic resistant genes (ARGs), as emerging environmental contaminants, have been reported to be accumulated in the soil environment. The use of NPs have raised increasing concerns about their environmental impacts, but the combined effect of NPs and antibiotics on ARGs remains less understood. Here, we established laboratory microcosms to explore the impacts of different concentrations of SiO2 NPs on β-lactam and sulfonamide resistance genes in soils amended with β-lactam or sulfonamide. Illumina sequencing and quantitative PCR revealed that the addition of NPs increased the bacterial community diversity but had no significant effects on the bacterial abundance. Moreover, NPs and sulfonamide jointly increased the abundances of sulfonamide resistance genes, while the exposure of NPs and β-lactam decreased β-lactam resistance genes. The detected ARGs were associated closely with two mobile genetic elements (MGEs, the tnpA and intI1 genes), indicating that MGEs may contribute to the dissemination of ARGs. Correlation analysis indicated the shifts in potential bacterial hosts and the frequency of horizontal gene transfer were important factors explaining the patterns of ARGs. Furthermore, structural equation models indicated that NPs exposure decreased the abundances of β-lactam resistance genes by driving changes in bacterial community and MGEs, whereas the increased abundances of sulfonamide resistance genes were mainly associated with the bacterial community, diversity and MGEs mediated by NPs and antibiotics. These results suggested that the combined effects of NPs and antibiotics on soil bacterial resistance were different due to the types of antibiotics. © 2020 The Author(s). Published by IOP Publishing Ltd.
英文关键词	antibiotic resistance genes; bacterial composition; mobile genetic element; SiO2 nanoparticles
语种	英语
scopus关键词	Antibiotics; Bacteria; DNA; Gene transfer; Genes; Nanoparticles; Polymerase chain reaction; Silica; Silica nanoparticles; Silicon oxides; SiO2 nanoparticles; Soils; Sulfur compounds; Antibiotic resistance genes; Antibiotic-resistant genes; Bacterial composition; Correlation analysis; Environmental contaminant; Genetic elements; Horizontal gene transfer; Structural equation models; Soil pollution; antibiotic resistance; antibiotics; bacterium; equation; gene transfer; nanoparticle; silicon; soil microorganism; Bacteria (microorganisms)
来源期刊	Environmental Research Letters
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/154188
作者单位	Institute of Loess Plateau, Shanxi University, Taiyuan, Shanxi, 030006, China; School of Environment and Resources, Shanxi University, Taiyuan, 030006, China; School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, University of MelbourneVictoria 3010, Australia
推荐引用方式 GB/T 7714	Zhang X.,Li J.,Li D.,et al. Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics[J],2020,15(3).
APA	Zhang X.,Li J.,Li D.,Zhang H.,&Hu H..(2020).Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics.Environmental Research Letters,15(3).
MLA	Zhang X.,et al."Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics".Environmental Research Letters 15.3(2020).