气候变化领域集成服务门户(CCPortal): Molecular methods for cost-efficient monitoring of HAB (harmful algal bloom) dinoflagellate resting cysts

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DOI	10.1016/j.marpolbul.2018.06.013
	Molecular methods for cost-efficient monitoring of HAB (harmful algal bloom) dinoflagellate resting cysts
	Perini F.; Bastianini M.; Capellacci S.; Pugliese L.; DiPoi E.; Cabrini M.; Buratti S.; Marini M.; Penna A.
发表日期	2019
ISSN	0025326X
起始页码	209
结束页码	218
卷号	147
英文摘要	Cyst abundance and identity are essential for understanding and predicting blooms, and for assessing the dispersal of toxic target dinoflagellate species by natural or human mediated ways, as with ballast waters. The aim of this study was to apply rapid, specific and sensitive qPCR assays to enumerate toxic dinoflagellate cysts in sediment samples collected from Adriatic harbours. The molecular standard curves of various target species allowed obtaining the rDNA copy number per cyst. The analytical sensitivity for specific standard curves was determined to be 2 or 10 rDNA copies per reaction. The abundance varied in the range of 1–747 dinoflagellate cysts g−1 dry weight. The assays showed greater sensitivity as compared to counts by light microscopy. This qPCR method revealed a powerful tool for the quantification of cysts from toxic dinoflagellate resting stages in sediment samples from Adriatic ports. © 2018 Elsevier Ltd
英文关键词	Adriatic Sea; Cysts; HAB species; Microscopy; Quantitative PCR; Sediments
语种	英语
scopus关键词	Microscopic examination; Polymerase chain reaction; Ports and harbors; Adriatic Sea; Cysts; Dinoflagellate cysts; HAB species; Harmful algal blooms; Molecular standards; Quantitative PCR; Toxic dinoflagellate; Sediments; algal bloom; dinoflagellate cyst; DNA; molecular analysis; polymerase chain reaction; quantitative analysis; toxicity; algal bloom; article; controlled study; cyst (resting stage); dry weight; human; human tissue; microscopy; monitoring; nonhuman; real time polymerase chain reaction; sediment; target organism; biological monitoring; copy number variation; dinoflagellate; genetics; Mediterranean Sea; microbiology; physiology; plasmid; procedures; real time polymerase chain reaction; reproducibility; season; sediment; sensitivity and specificity; ship; Adriatic Sea; Mediterranean Sea; algae; Dinophyceae; ribosome DNA; Biological Monitoring; Dinoflagellida; DNA Copy Number Variations; DNA, Ribosomal; Geologic Sediments; Harmful Algal Bloom; Mediterranean Sea; Plasmids; Real-Time Polymerase Chain Reaction; Reproducibility of Results; Seasons; Sensitivity and Specificity; Ships
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149636
作者单位	Department of Biomolecular Sciences, University of Urbino, Pesaro, Italy; ISMAR-CNR, Istituto di Scienze Marine, Consiglio Nazionale delle Ricerche, Venezia, Italy; OGS, Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Sgonico, Italy; Fondazione Centro Ricerche Marine, Cesenatico, Italy; ISMAR-CNR, Istituto di Scienze Marine, Consiglio Nazionale delle Ricerche, Ancona, Italy
推荐引用方式 GB/T 7714	Perini F.,Bastianini M.,Capellacci S.,et al. Molecular methods for cost-efficient monitoring of HAB (harmful algal bloom) dinoflagellate resting cysts[J],2019,147.
APA	Perini F..,Bastianini M..,Capellacci S..,Pugliese L..,DiPoi E..,...&Penna A..(2019).Molecular methods for cost-efficient monitoring of HAB (harmful algal bloom) dinoflagellate resting cysts.Marine Pollution Bulletin,147.
MLA	Perini F.,et al."Molecular methods for cost-efficient monitoring of HAB (harmful algal bloom) dinoflagellate resting cysts".Marine Pollution Bulletin 147(2019).