气候变化领域集成服务门户(CCPortal): Incidence of norovirus in tropical seafood harbouring fecal indicator bacteria

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DOI	10.1016/j.marpolbul.2019.110777
	Incidence of norovirus in tropical seafood harbouring fecal indicator bacteria
	Das O.; Lekshmi M.; Kumar S.; Nayak B.B.
发表日期	2020
ISSN	0025326X
卷号	150
英文摘要	The occurrence of human norovirus (HuNoV) in fresh seafood was studied by reverse transcription-polymerase chain reaction (RT-PCR). Samples (n = 104) comprising of oysters, clams, shrimps and finfish were collected from 3 retail markets, a landing center and a rocky intertidal zone. The samples were subjected to virus concentration, followed by RNA extraction and RT-PCR. Of these, 43 (41.34%) samples were positive for HuNoV Group II (GII). The fecal contamination status of the samples was determined by the Most Probable Number (MPN) method. All (100%) the samples were fecally contaminated, with the fecal coliform counts ranging from 10 to >1400 MPN/100 g. The study shows that fresh seafood, especially the bivalve mollusks, can act as vehicles for transmission and spread of HuNoV in the community. The high level of HuNoV contamination of seafood not only constitutes a health risk to the domestic consumers, but also compromises the microbiological qualities of seafood meant for international trade. © 2019 Elsevier Ltd
英文关键词	Fecal indicator; Norovirus; RT-PCR; Seafood; Shellfish
语种	英语
scopus关键词	Health risks; International trade; Meats; Molluscs; Shellfish; Tropics; Vehicle transmissions; Viruses; Fecal indicator bacteria; Fecal indicators; Microbiological quality; Most probable number methods; Norovirus; Reverse transcription-polymerase chain reaction; RT-PCR; Seafood; Polymerase chain reaction; bioaccumulation; bioindicator; disease incidence; fecal coliform; food quality; microbiology; polymerase chain reaction; seafood; shellfish; article; clam; consumer; contamination; fecal coliform; feces; health hazard; human; incidence; intertidal zone; major clinical study; most probable number method; nonhuman; Norovirus; oyster; reverse transcription polymerase chain reaction; RNA extraction; shellfish; shrimp; virus concentration; animal; bacterium; commercial phenomena; food control; incidence; international cooperation; sea food; Bivalvia; Mollusca; Norovirus; Ostreidae; Animals; Bacteria; Commerce; Food Microbiology; Humans; Incidence; Internationality; Norovirus; Seafood
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149339
作者单位	Post Harvest Technology, ICAR-Central Institute of Fisheries Education, Versova, Andheri(W), Mumbai, 400061, India
推荐引用方式 GB/T 7714	Das O.,Lekshmi M.,Kumar S.,et al. Incidence of norovirus in tropical seafood harbouring fecal indicator bacteria[J],2020,150.
APA	Das O.,Lekshmi M.,Kumar S.,&Nayak B.B..(2020).Incidence of norovirus in tropical seafood harbouring fecal indicator bacteria.Marine Pollution Bulletin,150.
MLA	Das O.,et al."Incidence of norovirus in tropical seafood harbouring fecal indicator bacteria".Marine Pollution Bulletin 150(2020).