气候变化领域集成服务门户(CCPortal): Vertical shifts of particle-attached and free-living prokaryotes in the water column above the cold seeps of the South China Sea

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DOI	10.1016/j.marpolbul.2020.111230
	Vertical shifts of particle-attached and free-living prokaryotes in the water column above the cold seeps of the South China Sea
	Zhang Y.; Jing H.; Peng X.
发表日期	2020
ISSN	0025326X
卷号	156
英文摘要	Marine particle-attached (PA) and free-living (FL) microbes play important roles in the biogeochemical cycling of organic matter along the water column. Deep-sea cold seeps are highly productive and chemosynthetic ecosystems, their continuous emission of CH4, CO2, and H2S can reach up to 100 m in the above water, therefore would influence the distribution and potential metabolic functions of deep-sea prokaryotes. In this study, the vertical distribution profiles of both PA and FL microbes in the water column above two cold seeps of the South China Sea were investigated using Illumina sequencing and quantitative PCR (qPCR) based on 16S rRNA gene. Photosynthetic and heterotrophic prokaryotes were predominant in respective surface and deep layers below the photic zone. The typical cold seep chemosynthetic microbes, such as methanotrophs and sulfate-reducing bacteria were observed with low proportions in the two cold seeps as well. Distinct PA and FL microbial fractions were found in terms of abundance and diversity. FL fraction exposed to the bulk water was significantly affected by temperature and inorganic nutrients, whereas PA fraction relied more on the organic matter of the particles and less susceptible to the environmental variability. Our study highlights the importance of vertical geochemical gradients on the distribution and potential metabolic choice of marine microbes and extends our current knowledge of depth-associated microbial distribution patterns. © 2020 The Authors
英文关键词	Cold seeps; Free-living; Particle-attached; Water column
语种	英语
scopus关键词	Biogeochemistry; Metabolism; Organic compounds; Polymerase chain reaction; RNA; Sulfur compounds; Biogeochemical cycling; Continuous emission; Environmental variability; Inorganic nutrients; Metabolic function; Microbial distribution; Sulfate reducing bacteria; Vertical distribution profiles; Bacteria; organic matter; RNA 16S; RNA 16S; water; abundance; biogeochemical cycle; carbon dioxide; chemosynthesis; cold seep; cold seep organism; dominance; hydrogen sulfide; methane; organic matter; polymerase chain reaction; prokaryote; quantitative analysis; species diversity; water column; 16S rRNA gene; Article; cold seep species; environmental temperature; geochemical analysis; Gram negative anaerobic bacteria; heterotrophy; high throughput sequencing; inorganic nutrient; methanotrophic bacterium; nonhuman; particle size; particulate matter; phototrophic bacterium; prokaryote; real time polymerase chain reaction; RNA sequence; sea pollution; sequence analysis; South China Sea; surface area; China; cold; ecosystem; Pacific Ocean; South China Sea; Prokaryota; China; Cold Temperature; Ecosystem; RNA, Ribosomal, 16S; Water
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/148939
作者单位	CAS Key Laboratory for Experimental Study under Deep-sea Extreme Conditions, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China; Southern Marine Science and Engineering Guangdong Laboratory (ZhuHai), China
推荐引用方式 GB/T 7714	Zhang Y.,Jing H.,Peng X.. Vertical shifts of particle-attached and free-living prokaryotes in the water column above the cold seeps of the South China Sea[J],2020,156.
APA	Zhang Y.,Jing H.,&Peng X..(2020).Vertical shifts of particle-attached and free-living prokaryotes in the water column above the cold seeps of the South China Sea.Marine Pollution Bulletin,156.
MLA	Zhang Y.,et al."Vertical shifts of particle-attached and free-living prokaryotes in the water column above the cold seeps of the South China Sea".Marine Pollution Bulletin 156(2020).