气候变化领域集成服务门户(CCPortal): Investigation of the toxic effects of different polystyrene micro-and nanoplastics on microalgae Chlorella vulgaris by analysis of cell viability; pigment content; oxidative stress and ultrastructural changes

CCPortal

DOI	10.1016/j.marpolbul.2020.111278
	Investigation of the toxic effects of different polystyrene micro-and nanoplastics on microalgae Chlorella vulgaris by analysis of cell viability; pigment content; oxidative stress and ultrastructural changes
	Hazeem L.J.; Yesilay G.; Bououdina M.; Perna S.; Cetin D.; Suludere Z.; Barras A.; Boukherroub R.
发表日期	2020
ISSN	0025326X
卷号	156
英文摘要	Plastics of different sizes (micro- and nano-sized) are often identified in aquatic environments. Nevertheless, their influence on marine organisms has not been widely investigated. In this study, the responses of the microalga Chlorella vulgaris to micro- and nanoplastics exposure were examined using long term toxicity test. The plastics tested were carboxyl-functionalized and non-functionalized polystyrene of 20, 50 and 500 nm in diameter. A reduction in algal cell viability and chlorophyll a concentration has been observed after exposure to the small sizes (20 and 50 nm) of plastics. Lactate dehydrogenase activity and reactive oxygen species concentration/production were significantly higher after exposure to the 20 nm nanoplastics than that of control confirming the stress condition. Fourier transform infrared (FTIR) spectroscopy analysis proved the attachment of nanoplastics to microalgae and rearrangement of extracellular polymeric substances. The cellular stress appeared as increased cell size, deformed cell wall and increased volume of starch grains. © 2020 Elsevier Ltd
英文关键词	Chlorella vulgaris; Cytotoxicity test; Microplastics; Nanoplastics; Ultrastructural changes
语种	英语
scopus关键词	Algae; Cytology; Elastomers; Fourier transform infrared spectroscopy; Marine biology; Microorganisms; Plastics; Polystyrenes; Aquatic environments; Carboxyl-functionalized; Chlorella vulgaris; Chlorophyll-a concentration; Extra-cellular polymeric substances; Lactate dehydrogenase activities; Pigment contents; Ultrastructural changes; Cells; chlorophyll a; lactate dehydrogenase; microplastic; nanoplastic; polystyrene derivative; reactive oxygen metabolite; chlorophyll a; polystyrene derivative; cell; microalga; oxidative stress; pigment; plastic waste; polymer; toxicity; ultrastructure; viability; Article; cell viability; Chlorella vulgaris; controlled study; enzyme activity; exposure; Fourier transform infrared spectroscopy; nonhuman; oxidative stress; reduction (chemistry); scanning electron microscopy; toxic concentration; toxicity; toxicity testing; transmission electron microscopy; cell survival; Chlorella vulgaris; microalga; oxidative stress; water pollutant; algae; Chlorella vulgaris; Cell Survival; Chlorella vulgaris; Chlorophyll A; Microalgae; Oxidative Stress; Polystyrenes; Water Pollutants, Chemical
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/148884
作者单位	Department of Biology, College of Science, University of Bahrain3203, Bahrain; Molecular Biology and Genetics Department, Hamidiye Institute of Health Sciences, University of Health Sciences, Istanbul, 34668, Turkey; Department of Physics, College of Science, University of Bahrain3203, Bahrain; Department of Mathematics and Science Education, Gazi Faculty of Education, Gazi University, Ankara, 06500, Turkey; Department of Biology, Faculty of Science, Gazi University, Ankara, 06500, Turkey; Univ. Lille, CNRS, Central Lille, ISEN, Univ. Polytechnique Hauts-de-France, UMR 8520, IEMN, Lille, F-59000, France
推荐引用方式 GB/T 7714	Hazeem L.J.,Yesilay G.,Bououdina M.,et al. Investigation of the toxic effects of different polystyrene micro-and nanoplastics on microalgae Chlorella vulgaris by analysis of cell viability; pigment content; oxidative stress and ultrastructural changes[J],2020,156.
APA	Hazeem L.J..,Yesilay G..,Bououdina M..,Perna S..,Cetin D..,...&Boukherroub R..(2020).Investigation of the toxic effects of different polystyrene micro-and nanoplastics on microalgae Chlorella vulgaris by analysis of cell viability; pigment content; oxidative stress and ultrastructural changes.Marine Pollution Bulletin,156.
MLA	Hazeem L.J.,et al."Investigation of the toxic effects of different polystyrene micro-and nanoplastics on microalgae Chlorella vulgaris by analysis of cell viability; pigment content; oxidative stress and ultrastructural changes".Marine Pollution Bulletin 156(2020).