气候变化领域集成服务门户(CCPortal): Production; characterization; evaluation and toxicity assessment of a Bacillus cereus UCP 1615 biosurfactant for marine oil spills bioremediation

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DOI	10.1016/j.marpolbul.2020.111357
	Production; characterization; evaluation and toxicity assessment of a Bacillus cereus UCP 1615 biosurfactant for marine oil spills bioremediation
	Durval I.J.B.; Mendonça A.H.R.; Rocha I.V.; Luna J.M.; Rufino R.D.; Converti A.; Sarubbo L.A.
发表日期	2020
ISSN	0025326X
卷号	157
英文摘要	In this study, Bacillus cereus was cultivated in a mineral medium composed of 2% frying oil and 0.12% peptone to produce a biosurfactant. The production was scaled up from flasks to 1.2-, 3.0- and 50-L bioreactors, where surface tension achieved 28.7, 27.5 and 32 mN/m and biosurfactant concentration 4.3, 4.6 and 4.7 g/L, respectively. The biosurfactant was characterized as anionic, while nuclear magnetic resonance, thin-layer chromatography and gas chromatography analyses revealed its lipopeptide nature. Toxicity tests showed survival rates of the fish Poecilia vivipara and the bivalve Anomalocardia brasiliana higher than 90% and 55%, respectively, thus suggesting the use of this biosurfactant in marine environment depollution. Moreover, the biosurfactant stimulated the growth of autochthonous microorganisms independently of the presence of motor oil in bioassays performed in seawater. These results demonstrate that the biosurfactant is biocompatible and has potential for industrial-scale production and application to bioremediation of oil spills-polluted marine environment. © 2020 Elsevier Ltd
英文关键词	Anomalocardia brasiliana; Bacillus cereus; Bioremediation; Biosurfactant; Poecilia vivipara; Toxicity
语种	英语
scopus关键词	Bacillus cereus; Bacteriology; Biocompatibility; Biomolecules; Bioremediation; Biotechnology; Gas chromatography; Oil spills; Surface active agents; Thin layer chromatography; Toxicity; Autochthonous microorganisms; Bio surfactant; Gas chromatography analysis; Industrial-scale production; Marine environment; Marine oil spills; Toxicity assessment; Toxicity test; Marine pollution; biosurfactant; peptone; petroleum; surfactant; bacterium; bioremediation; bivalve; cyprinid; marine pollution; oil spill; survival; toxicity test; Anomalocardia brasiliana; Article; Bacillus cereus; biocompatibility; bioremediation; bivalve; controlled study; gas chromatography; microbial growth; nonhuman; nuclear magnetic resonance; oil spill; Poecilia; Poecilia vivipara; scale up; surface tension; survival rate; thin layer chromatography; toxicity testing; Bacillus cereus; bioremediation; Anomalocardia brasiliana; Bacillus cereus; Bivalvia; Poecilia vivipara; Bacillus cereus; Biodegradation, Environmental; Petroleum; Petroleum Pollution; Surface-Active Agents
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/148832
作者单位	Northeast Biotechnology Network (RENORBIO), Federal Rural University of Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, Recife, PE 52171-900, Brazil; Catholic University of Pernambuco, Rua do Príncipe, n. 526, Boa Vista, Recife, PE 50050-900, Brazil; Advanced Institute of Technology and Innovation (IATI), Rua Joaquim de Brito, n. 216, Boa Vista, Recife, PE 50070-280, Brazil; Department of Microbiology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, CPqAM/Fiocruz, Av. Professor Moraes Rego, s/n, Cidade Universitária, Recife, PE 50670-420, Brazil; Department of Civil, Chemical and Environmental Engineering, Genoa University, Pole of Chemical Engineering, Via Opera Pia 15, Genova, I-16145, Italy
推荐引用方式 GB/T 7714	Durval I.J.B.,Mendonça A.H.R.,Rocha I.V.,et al. Production; characterization; evaluation and toxicity assessment of a Bacillus cereus UCP 1615 biosurfactant for marine oil spills bioremediation[J],2020,157.
APA	Durval I.J.B..,Mendonça A.H.R..,Rocha I.V..,Luna J.M..,Rufino R.D..,...&Sarubbo L.A..(2020).Production; characterization; evaluation and toxicity assessment of a Bacillus cereus UCP 1615 biosurfactant for marine oil spills bioremediation.Marine Pollution Bulletin,157.
MLA	Durval I.J.B.,et al."Production; characterization; evaluation and toxicity assessment of a Bacillus cereus UCP 1615 biosurfactant for marine oil spills bioremediation".Marine Pollution Bulletin 157(2020).