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DOI	10.1016/j.marpolbul.2020.111601
	Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka; Oryzias latipes
	Kato-Unoki Y.; Takai Y.; Nagano Y.; Matsunaga S.; Enoki S.; Takamura T.; Kim S.; Kinoshita M.; Kitano T.; Shimasaki Y.; Oshima Y.
发表日期	2020
ISSN	0025326X
卷号	160
英文摘要	Tributyltin-binding proteins (TBT-bps), members of the lipocalin family, bind TBT in fish blood and are presumed to contribute to detoxification of TBT. Recent studies have shown that many fish species have TBT-bp genes, and that these genes are induced by stresses such as exposure to chemicals or fish pathogenic bacteria. However, the function of TBT-bps, and the mechanisms of their induction and detoxification activity are still unclear. Here, towards elucidating the functions of TBT-bp2, we produced a TBT-bp2 knockout (TBT-bp2−/−) strain of Japanese medaka, Oryzias latipes, by using the CRISPR/Cas9 system. Gene expression of the mutated TBT-bp2 was reduced, and the cDNA sequencing and predicted protein structure suggested possible loss of function. However, the fish could be grown under normal conditions. Exposure of the TBT-bp2−/− strain of medaka to various stresses in future experiments is expected to contribute to our understanding of this novel detoxification system in aquatic organisms. © 2020 Elsevier Ltd
英文关键词	CRISPR/Cas9; Detoxification; Knockout; Oryzias latipes; Tributyltin-binding protein2 (TBT-bp2)
语种	英语
scopus关键词	Bacteria; Detoxification; Gene expression; Insecticides; Proteins; Binding proteins; Fish pathogenic bacterium; Fish species; Japanese medaka; Knockout mutant; Normal condition; Oryzias latipes; Protein structures; Fish; lipocalin; tributyltin binding protein 2; unclassified drug; carrier protein; organotin compound; tributyltin; biotechnology; detoxification; finfish; gene expression; pathogen; protein; tributyltin; adult; animal experiment; aquatic species; Article; controlled study; CRISPR-CAS9 system; detoxification; embryo; environmental exposure; environmental stress; female; growth; male; mutant; mutation; nonhuman; Oryzias latipes; protein expression; protein function; protein synthesis; animal; genetics; Oryzias; Oryzias latipes; Oryziinae; Animals; Carrier Proteins; Oryzias; Trialkyltin Compounds
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/148594
作者单位	Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan; Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan; Chemicals Evaluation and Research Institute, Japan, 3-2-7, Miyanojin, Kurume-shi, Fukuoka, 839-0801, Japan; Laboratory of Molecular Gene Technology, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan; Department of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan; Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, 860-8555, Japan; Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa, 920-1192, Japan
推荐引用方式 GB/T 7714	Kato-Unoki Y.,Takai Y.,Nagano Y.,et al. Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka; Oryzias latipes[J],2020,160.
APA	Kato-Unoki Y..,Takai Y..,Nagano Y..,Matsunaga S..,Enoki S..,...&Oshima Y..(2020).Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka; Oryzias latipes.Marine Pollution Bulletin,160.
MLA	Kato-Unoki Y.,et al."Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka; Oryzias latipes".Marine Pollution Bulletin 160(2020).