气候变化领域集成服务门户(CCPortal): Quantitative immunodetection of metalloprotein II in Perinereis aibuhitensis after heavy metal exposure

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DOI	10.1016/j.marpolbul.2020.111550
	Quantitative immunodetection of metalloprotein II in Perinereis aibuhitensis after heavy metal exposure
	Zhao H.; Wang M.; Pang M.; Sun J.; Huang Y.; Gao F.; Yang D.; Zhou Y.; Wang B.
发表日期	2020
ISSN	0025326X
卷号	160
英文摘要	The metal detoxification mechanism is essential for the accumulation activity of some polychaetes. In order to investigate the detoxification function of metalloprotein II in polychaete Perinereis aibuhitensis, a recombinant fusion protein of MP II was successfully expressed in vitro and an anti-MP II polyclonal antibody was got. The tissue distribution of MP II in P. aibuhitensis and the protein expression under Cd, Pb and Zn exposure were detected by ELISA with this antibody. The results showed that MP II was higher in the intestine of P. aibuhitensis, followed by the body wall and parapodium. Cd, Zn and Pb exposure can induced MP II expression, but the change trend of MP II under various heavy metal exposure was different. The amount of MP II induced in P. aibuhitensis increased with the rises of Cd concentration, but there is no significant relationship between Zn and Pb concentration and MP II level. © 2020 Elsevier Ltd
英文关键词	Metal exposure; MP II; Perinereis aibuhitensis; Protein expression; Recombinant expression; Tissue distribution
语种	英语
scopus关键词	Antibodies; Detoxification; Lead; Plants (botany); Recombinant proteins; Zinc; Accumulation activity; Cd concentrations; Fusion proteins; Metal detoxification; Pb concentration; Polyclonal antibody; Protein expressions; Tissue distributions; Cadmium compounds; cadmium; heavy metal; lead; metalloprotein; metalloprotein II; unclassified drug; zinc; metalloprotein; concentration (composition); detection method; detoxification; gene expression; heavy metal; pollution exposure; protein; qualitative analysis; Article; controlled study; detoxification; environmental exposure; enzyme linked immunosorbent assay; immunodetection; in vitro study; nonhuman; Perinereis aibuhitensis; Polychaeta; protein analysis; protein expression; trend study; animal; Polychaeta; Perinereis aibuhitensis; Polychaeta; Animals; Metalloproteins; Metals, Heavy; Polychaeta
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/148589
作者单位	Key laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China; Key Laboratory of Science and Engineering for the Marine Ecological Environment, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China
推荐引用方式 GB/T 7714	Zhao H.,Wang M.,Pang M.,et al. Quantitative immunodetection of metalloprotein II in Perinereis aibuhitensis after heavy metal exposure[J],2020,160.
APA	Zhao H..,Wang M..,Pang M..,Sun J..,Huang Y..,...&Wang B..(2020).Quantitative immunodetection of metalloprotein II in Perinereis aibuhitensis after heavy metal exposure.Marine Pollution Bulletin,160.
MLA	Zhao H.,et al."Quantitative immunodetection of metalloprotein II in Perinereis aibuhitensis after heavy metal exposure".Marine Pollution Bulletin 160(2020).