气候变化领域集成服务门户(CCPortal): Sequencing metabolically labeled transcripts in single cells reveals mRNA turnover strategies

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DOI	10.1126/science.abb6502
	Sequencing metabolically labeled transcripts in single cells reveals mRNA turnover strategies
	Battich N.; Beumer J.; De Barbanson B.; Krenning L.; Baron C.S.; Tanenbaum M.E.; Clevers H.; Van Oudenaarden A.
发表日期	2020
ISSN	0036-8075
起始页码	1151
结束页码	1156
卷号	367 期号:6483
英文摘要	The regulation of messenger RNA levels in mammalian cells can be achieved by the modulation of synthesis and degradation rates. Metabolic RNA-labeling experiments in bulk have quantified these rates using relatively homogeneous cell populations. However, to determine these rates during complex dynamical processes, for instance during cellular differentiation, single-cell resolution is required. Therefore, we developed a method that simultaneously quantifies metabolically labeled and preexisting unlabeled transcripts in thousands of individual cells. We determined synthesis and degradation rates during the cell cycle and during differentiation of intestinal stem cells, revealing major regulatory strategies. These strategies have distinct consequences for controlling the dynamic range and precision of gene expression. These findings advance our understanding of how individual cells in heterogeneous populations shape their gene expression dynamics. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	messenger RNA dyes, reagents, indicators, markers and buffers messenger RNA uridine cell component differentiation gene expression metabolism RNA turnover Article cell cycle cell differentiation cell metabolism gene cluster gene control gene expression genetic transcription human intestinal stem cell molecular dynamics priority journal regulatory mechanism RNA sequencing single cell analysis animal chemistry K-562 cell line metabolism mouse procedures RNA stability sequence analysis single cell analysis Mammalia Animals Humans Indicators and Reagents K562 Cells Mice RNA Stability RNA, Messenger Sequence Analysis, RNA Single-Cell Analysis Transcription, Genetic Uridine
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133644
推荐引用方式 GB/T 7714	Battich N.,Beumer J.,De Barbanson B.,et al. Sequencing metabolically labeled transcripts in single cells reveals mRNA turnover strategies[J]. Science,2020,367(6483).
APA	Battich N..,Beumer J..,De Barbanson B..,Krenning L..,Baron C.S..,...&Van Oudenaarden A..(2020).Sequencing metabolically labeled transcripts in single cells reveals mRNA turnover strategies.,367(6483).
MLA	Battich N.,et al."Sequencing metabolically labeled transcripts in single cells reveals mRNA turnover strategies".367.6483(2020).