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DOI	10.1126/science.abb7498
	Structure of the RNA-dependent RNA polymerase from COVID-19 virus
	Gao Y.; Yan L.; Huang Y.; Liu F.; Zhao Y.; Cao L.; Wang T.; Sun Q.; Ming Z.; Zhang L.; Ge J.; Zheng L.; Zhang Y.; Wang H.; Zhu Y.; Zhu C.; Hu T.; Hua T.; Zhang B.; Yang X.; Li J.; Yang H.; Liu Z.; Xu W.; Guddat L.W.; Wang Q.; Lou Z.; Rao Z.
发表日期	2020
ISSN	0036-8075
起始页码	779
结束页码	782
卷号	368 期号:6492
英文摘要	A novel coronavirus [severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2)] outbreak has caused a global coronavirus disease 2019 (COVID-19) pandemic, resulting in tens of thousands of infections and thousands of deaths worldwide. The RNA-dependent RNA polymerase [(RdRp), also named nsp12] is the central component of coronaviral replication and transcription machinery, and it appears to be a primary target for the antiviral drug remdesivir. We report the cryo–electron microscopy structure of COVID-19 virus full-length nsp12 in complex with cofactors nsp7 and nsp8 at 2.9-angstrom resolution. In addition to the conserved architecture of the polymerase core of the viral polymerase family, nsp12 possesses a newly identified b-hairpin domain at its N terminus. A comparative analysis model shows how remdesivir binds to this polymerase. The structure provides a basis for the design of new antiviral therapeutics that target viral RdRp. Copyright © 2020 The Authors,
关键词	comparative study drug electron microscopy enzyme enzyme activity RNA virus Coronavirus SARS coronavirus adenosine phosphate alanine antivirus agent NSP7 protein, SARS-CoV-2 NSP8 protein, SARS-CoV-2 remdesivir RNA directed RNA polymerase RNA-dependent RNA polymerase, coronavirus viral protein beta sheet Betacoronavirus chemistry cryoelectron microscopy drug design enzyme active site enzymology metabolism molecular model protein domain ultrastructure Adenosine Monophosphate Alanine Antiviral Agents Betacoronavirus Catalytic Domain Cryoelectron Microscopy Drug Design Models, Molecular Protein Conformation, beta-Strand Protein Domains RNA Replicase Viral Nonstructural Proteins
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133506
推荐引用方式 GB/T 7714	Gao Y.,Yan L.,Huang Y.,et al. Structure of the RNA-dependent RNA polymerase from COVID-19 virus[J]. Science,2020,368(6492).
APA	Gao Y..,Yan L..,Huang Y..,Liu F..,Zhao Y..,...&Rao Z..(2020).Structure of the RNA-dependent RNA polymerase from COVID-19 virus.,368(6492).
MLA	Gao Y.,et al."Structure of the RNA-dependent RNA polymerase from COVID-19 virus".368.6492(2020).