气候变化领域集成服务门户(CCPortal): Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells

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DOI	10.1126/science.aay9207
	Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells
	Bálint Š.; Müller S.; Fischer R.; Kessler B.M.; Harkiolaki M.; Valitutti S.; Dustin M.L.
发表日期	2020
ISSN	0036-8075
起始页码	897
结束页码	901
卷号	368 期号:6493
英文摘要	Cytotoxic T lymphocytes (CTLs) kill infected and cancerous cells. We detected transfer of cytotoxic multiprotein complexes, called supramolecular attack particles (SMAPs), from CTLs to target cells. SMAPs were rapidly released from CTLs and were autonomously cytotoxic. Mass spectrometry, immunochemical analysis, and CRISPR editing identified a carboxyl-terminal fragment of thrombospondin-1 as an unexpected SMAP component that contributed to target killing. Direct stochastic optical reconstruction microscopy resolved a cytotoxic core surrounded by a thrombospondin-1 shell of ~120 nanometer diameter. Cryo-soft x-ray tomography analysis revealed that SMAPs had a carbon-dense shell and were stored in multicore granules. We propose that SMAPs are autonomous extracellular killing entities that deliver cytotoxic cargo targeted by the specificity of shell components. Copyright © 2020 The Authors.
关键词	cancer cell identification method molecular analysis reconstruction granzyme multiprotein complex perforin thrombospondin 1 CRISPR Cas system cytotoxic T lymphocyte cytotoxicity exocytosis gene editing genetics human K-562 cell line metabolism x-ray tomography CRISPR-Cas Systems Cytotoxicity, Immunologic Exocytosis Gene Editing Granzymes Humans K562 Cells Multiprotein Complexes Perforin T-Lymphocytes, Cytotoxic Thrombospondin 1 Tomography, X-Ray
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133482
推荐引用方式 GB/T 7714	Bálint Š.,Müller S.,Fischer R.,et al. Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells[J]. Science,2020,368(6493).
APA	Bálint Š..,Müller S..,Fischer R..,Kessler B.M..,Harkiolaki M..,...&Dustin M.L..(2020).Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells.,368(6493).
MLA	Bálint Š.,et al."Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells".368.6493(2020).