气候变化领域集成服务门户(CCPortal): The histone H3-H4 tetramer is a copper reductase enzyme

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DOI	10.1126/science.aba8740
	The histone H3-H4 tetramer is a copper reductase enzyme
	Attar N.; Campos O.A.; Vogelauer M.; Cheng C.; Xue Y.; Schmollinger S.; Salwinski L.; Mallipeddi N.V.; Boone B.A.; Yen L.; Yang S.; Zikovich S.; Dardine J.; Carey M.F.; Merchant S.S.; Kurdistani S.K.
发表日期	2020
ISSN	10959203
起始页码	59
结束页码	64
卷号	369 期号:6499
英文摘要	Eukaryotic histone H3-H4 tetramers contain a putative copper (Cu2+) binding site at the H3-H3' dimerization interface with unknown function. The coincident emergence of eukaryotes with global oxygenation, which challenged cellular copper utilization, raised the possibility that histones may function in cellular copper homeostasis. We report that the recombinant Xenopus laevis H3-H4 tetramer is an oxidoreductase enzyme that binds Cu2+ and catalyzes its reduction to Cu1+ in vitro. Loss- and gain-of-function mutations of the putative active site residues correspondingly altered copper binding and the enzymatic activity, as well as intracellular Cu1+ abundance and copper-dependent mitochondrial respiration and Sod1 function in the yeast Saccharomyces cerevisiae The histone H3-H4 tetramer, therefore, has a role other than chromatin compaction or epigenetic regulation and generates biousable Cu1+ ions in eukaryotes. Copyright © 2020, American Association for the Advancement of Science.
关键词	copper copper oxidase copper zinc superoxide dismutase histone MAC1 protein, S cerevisiae nuclear protein oxidoreductase recombinant protein Saccharomyces cerevisiae protein transcription factor animal biocatalysis chemistry enzyme active site enzymology gain of function mutation genetics metabolism mitochondrion protein multimerization Saccharomyces cerevisiae Xenopus laevis Animals Biocatalysis Catalytic Domain Copper Gain of Function Mutation Histones Mitochondria Nuclear Proteins Oxidoreductases Protein Multimerization Recombinant Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Superoxide Dismutase-1 Transcription Factors Xenopus laevis
语种	英语
来源机构	Science (New York, N.Y.)
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133378
推荐引用方式 GB/T 7714	Attar N.,Campos O.A.,Vogelauer M.,et al. The histone H3-H4 tetramer is a copper reductase enzyme[J]. Science (New York, N.Y.),2020,369(6499).
APA	Attar N..,Campos O.A..,Vogelauer M..,Cheng C..,Xue Y..,...&Kurdistani S.K..(2020).The histone H3-H4 tetramer is a copper reductase enzyme.,369(6499).
MLA	Attar N.,et al."The histone H3-H4 tetramer is a copper reductase enzyme".369.6499(2020).