气候变化领域集成服务门户(CCPortal): A combined lipidomic and 16S rRNA gene amplicon sequencing approach reveals archaeal sources of intact polar lipids in the stratified Black Sea water column

CCPortal

DOI	10.1111/gbi.12316
	A combined lipidomic and 16S rRNA gene amplicon sequencing approach reveals archaeal sources of intact polar lipids in the stratified Black Sea water column
	Sollai M.; Villanueva L.; Hopmans E.C.; Reichart G.-J.; Sinninghe Damsté J.S.
发表日期	2019
ISSN	14724677
起始页码	91
结束页码	109
卷号	17 期号:1
英文摘要	Archaea are important players in marine biogeochemical cycles, and their membrane lipids are useful biomarkers in environmental and geobiological studies. However, many archaeal groups remain uncultured and their lipid composition unknown. Here, we aim to expand the knowledge on archaeal lipid biomarkers and determine the potential sources of those lipids in the water column of the euxinic Black Sea. The archaeal community was evaluated by 16S rRNA gene amplicon sequencing and by quantitative PCR. The archaeal intact polar lipids (IPLs) were investigated by ultra-high-pressure liquid chromatography coupled to high-resolution mass spectrometry. Our study revealed both a complex archaeal community and large changes with water depth in the IPL assemblages. In the oxic/upper suboxic waters (<105 m), the archaeal community was dominated by marine group (MG) I Thaumarchaeota, coinciding with a higher relative abundance of hexose phosphohexose crenarchaeol, a known marker for Thaumarchaeota. In the suboxic waters (80–110 m), MGI Nitrosopumilus sp. dominated and produced predominantly monohexose glycerol dibiphytanyl glycerol tetraethers (GDGTs) and hydroxy-GDGTs. Two clades of MGII Euryarchaeota were present in the oxic and upper suboxic zones in much lower abundances, preventing the detection of their specific IPLs. In the deep sulfidic waters (>110 m), archaea belonging to the DPANN Woesearchaeota, Bathyarchaeota, and ANME-1b clades dominated. Correlation analyses suggest that the IPLs GDGT-0, GDGT-1, and GDGT-2 with two phosphatidylglycerol (PG) head groups and archaeol with a PG, phosphatidylethanolamine, and phosphatidylserine head groups were produced by ANME-1b archaea. Bathyarchaeota represented 55% of the archaea in the deeper part of the euxinic zone and likely produces archaeol with phospho-dihexose and hexose-glucuronic acid head groups. © 2018 The Authors. Geobiology Published by John Wiley & Sons Ltd
关键词	biogeochemistry biomarker genetic analysis high pressure lipid microbial community microbial ecology RNA suboxic conditions water column water depth Black Sea Archaea Candidatus Nitrosopumilus Euryarchaeota bacterial RNA lipid RNA 16S sea water archaeon Black Sea chemistry metabolism Archaea Black Sea Lipids RNA, Bacterial RNA, Ribosomal, 16S Seawater
语种	英语
来源机构	Geobiology
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133253
推荐引用方式 GB/T 7714	Sollai M.,Villanueva L.,Hopmans E.C.,et al. A combined lipidomic and 16S rRNA gene amplicon sequencing approach reveals archaeal sources of intact polar lipids in the stratified Black Sea water column[J]. Geobiology,2019,17(1).
APA	Sollai M.,Villanueva L.,Hopmans E.C.,Reichart G.-J.,&Sinninghe Damsté J.S..(2019).A combined lipidomic and 16S rRNA gene amplicon sequencing approach reveals archaeal sources of intact polar lipids in the stratified Black Sea water column.,17(1).
MLA	Sollai M.,et al."A combined lipidomic and 16S rRNA gene amplicon sequencing approach reveals archaeal sources of intact polar lipids in the stratified Black Sea water column".17.1(2019).