气候变化领域集成服务门户(CCPortal): Reconstructing the evolutionary history of nitrogenases: Evidence for ancestral molybdenum-cofactor utilization

CCPortal

DOI	10.1111/gbi.12381
	Reconstructing the evolutionary history of nitrogenases: Evidence for ancestral molybdenum-cofactor utilization
	Garcia A.K.; McShea H.; Kolaczkowski B.; Kaçar B.
发表日期	2020
ISSN	14724677
起始页码	394
结束页码	411
卷号	18 期号:3
英文摘要	The nitrogenase metalloenzyme family, essential for supplying fixed nitrogen to the biosphere, is one of life's key biogeochemical innovations. The three forms of nitrogenase differ in their metal dependence, each binding either a FeMo-, FeV-, or FeFe-cofactor where the reduction of dinitrogen takes place. The history of nitrogenase metal dependence has been of particular interest due to the possible implication that ancient marine metal availabilities have significantly constrained nitrogenase evolution over geologic time. Here, we reconstructed the evolutionary history of nitrogenases, and combined phylogenetic reconstruction, ancestral sequence inference, and structural homology modeling to evaluate the potential metal dependence of ancient nitrogenases. We find that active-site sequence features can reliably distinguish extant Mo-nitrogenases from V- and Fe-nitrogenases and that inferred ancestral sequences at the deepest nodes of the phylogeny suggest these ancient proteins most resemble modern Mo-nitrogenases. Taxa representing early-branching nitrogenase lineages lack one or more biosynthetic nifE and nifN genes that both contribute to the assembly of the FeMo-cofactor in studied organisms, suggesting that early Mo-nitrogenases may have utilized an alternate and/or simplified pathway for cofactor biosynthesis. Our results underscore the profound impacts that protein-level innovations likely had on shaping global biogeochemical cycles throughout the Precambrian, in contrast to organism-level innovations that characterize the Phanerozoic Eon. © 2020 The Authors. Geobiology Published by John Wiley & Sons Ltd
关键词	biogeochemistry chemical binding enzyme evolutionary biology gene homology molybdenum nitrogen fixation nutrient availability phylogenetics protein reconstruction
语种	英语
来源机构	Geobiology
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133187
推荐引用方式 GB/T 7714	Garcia A.K.,McShea H.,Kolaczkowski B.,et al. Reconstructing the evolutionary history of nitrogenases: Evidence for ancestral molybdenum-cofactor utilization[J]. Geobiology,2020,18(3).
APA	Garcia A.K.,McShea H.,Kolaczkowski B.,&Kaçar B..(2020).Reconstructing the evolutionary history of nitrogenases: Evidence for ancestral molybdenum-cofactor utilization.,18(3).
MLA	Garcia A.K.,et al."Reconstructing the evolutionary history of nitrogenases: Evidence for ancestral molybdenum-cofactor utilization".18.3(2020).