气候变化领域集成服务门户(CCPortal): Bacteria-in-paper, a versatile platform to study bacterial ecology

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DOI	10.1111/ele.13274
	Bacteria-in-paper, a versatile platform to study bacterial ecology
	Hol F.J.H.; Whitesides G.M.; Dekker C.
发表日期	2019
ISSN	1461023X
卷号	22 期号:8
英文摘要	Habitat spatial structure has a profound influence on bacterial life, yet there currently are no low-cost equipment-free laboratory techniques to reproduce the intricate structure of natural bacterial habitats. Here, we demonstrate the use of paper scaffolds to create landscapes spatially structured at the scales relevant to bacterial ecology. In paper scaffolds, planktonic bacteria migrate through liquid-filled pores, while the paper’s cellulose fibres serve as anchor points for sessile colonies (biofilms). Using this novel approach, we explore bacterial colonisation dynamics in different landscape topographies and characterise the community composition of Escherichia coli strains undergoing centimetre-scale range expansions in habitats structured at the micrometre scale. The bacteria-in-paper platform enables quantitative assessment of bacterial community dynamics in complex environments using everyday materials. © 2019 The Authors. Ecology Letters published by CNRS and John Wiley & Sons Ltd.
英文关键词	Bacteria; colonisation; dendritic networks; experimental tools; habitat structure; microfabrication; paper; population dynamics; range expansion; spatial structure
语种	英语
scopus关键词	bacterium; cellulose; coliform bacterium; colonization; community dynamics; ecology; equipment; experimental study; habitat structure; laboratory method; network analysis; population dynamics; range expansion; spatial analysis; Bacteria (microorganisms); Escherichia coli; bacterium; biofilm; ecology; ecosystem; Escherichia coli; growth, development and aging; paper; population dynamics; Bacteria; Biofilms; Ecology; Ecosystem; Escherichia coli; Paper; Population Dynamics
来源期刊	Ecology Letters
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/121063
作者单位	Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, United States; Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, Delft, HZ, 2629, Netherlands; Department of Bioengineering, Stanford University, 443 Via Ortega, Stanford, CA 94305, United States; Wyss Institute for Biologically Inspired Engineering, Harvard University, 60 Oxford Street, Cambridge, MA 02138, United States
推荐引用方式 GB/T 7714	Hol F.J.H.,Whitesides G.M.,Dekker C.. Bacteria-in-paper, a versatile platform to study bacterial ecology[J],2019,22(8).
APA	Hol F.J.H.,Whitesides G.M.,&Dekker C..(2019).Bacteria-in-paper, a versatile platform to study bacterial ecology.Ecology Letters,22(8).
MLA	Hol F.J.H.,et al."Bacteria-in-paper, a versatile platform to study bacterial ecology".Ecology Letters 22.8(2019).