气候变化领域集成服务门户(CCPortal): Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy

CCPortal

DOI	10.1038/s41467-019-11017-y
	Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy
	Wang, Changsheng; Tang, Shican; Zhan, Qilin; Hou, Qingqing; Zhao, Yan; Zhao, Qiang; Feng, Qi; Zhou, Congcong; Lyu, Danfeng; Cui, Lingling; Li, Yan; Miao, Jiashun; Zhu, Chuanrang; Lu, Yiqi; Wang, Yongchun; Wang, Ziqun; Zhu, Jingjie; Shangguan, Yingying; Gong, Junyi; Yang, Shihua; Wang, Wuqi; Zhang, Jianfu; Xie, Huaan; Huang, Xuehui; Han, Bin
发表日期	2019
ISSN	2041-1723
卷号	10
英文摘要	Hybrid rice breeding for exploiting hybrid vigor, heterosis, has greatly increased grain yield. However, the heterosis-related genes associated with rice grain production remain largely unknown, partly because comprehensive mapping of heterosis-related traits is still labor-intensive and time-consuming. Here, we present a quantitative trait locus (QTL) mapping method, GradedPool-Seq, for rapidly mapping QTLs by whole-genome sequencing of gradedpool samples from F-2 progeny via bulked-segregant analysis. We implement this method and map-based cloning to dissect the heterotic QTL GW3p6 from the female line. We then generate the near isogenic line NIL-FH676::GW3p6 by introgressing the GW3p6 allele from the female line Guangzhan63-4S into the male inbred line Fuhui676. The NIL-FH676::GW3p6 exhibits grain yield highly increased compared to Fuhui676. This study demonstrates that it may be possible to achieve a high level of grain production in inbred rice lines without the need to construct hybrids.
学科领域	Science & Technology - Other Topics
语种	英语
WOS研究方向	Multidisciplinary Sciences
来源期刊	NATURE COMMUNICATIONS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/112077
作者单位	Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Ctr Gene Res, CAS Ctr Excellence Mol Plant Sci,Inst Plant Physi, Shanghai 200233, Peoples R China
推荐引用方式 GB/T 7714	Wang, Changsheng,Tang, Shican,Zhan, Qilin,et al. Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy[J]. 中国科学院西北生态环境资源研究院,2019,10.
APA	Wang, Changsheng.,Tang, Shican.,Zhan, Qilin.,Hou, Qingqing.,Zhao, Yan.,...&Han, Bin.(2019).Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy.NATURE COMMUNICATIONS,10.
MLA	Wang, Changsheng,et al."Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy".NATURE COMMUNICATIONS 10(2019).