气候变化领域集成服务门户(CCPortal): The propensity for tumorigenesis in human induced pluripotent stem cells is related with genomic instability

CCPortal

DOI	10.5732/cjc.012.10065
	The propensity for tumorigenesis in human induced pluripotent stem cells is related with genomic instability
	Liang, Yi; Zhang, Hui; Feng, Qi-Sheng; Cai, Man-Bo; Deng, Wen; Qin, Dajiang; Yun, Jing-Ping; Tsao, George Sai Wah; Kang, Tiebang; Esteban, Miguel Angel; Pei, Duanqing; Zeng, Yi-Xin
发表日期	2013
ISSN	1000-467X
EISSN	1944-446X
卷号	32 期号:4
英文摘要	The discovery of induced pluripotent stem cells (iPSCs) is a promising advancement in the field of regenerative medicine. Previous studies have indicated that the teratoma-forming propensity of iPSCs is variable; however, the relationship between tumorigenic potential and genomic instability in human iPSCs (HiPSCs) remains to be fully elucidated. Here, we evaluated the malignant potential of HiPSCs by using both colony formation assays and tumorigenicity tests. We demonstrated that HiPSCs formed tumorigenic colonies when grown in cancer cell culture medium and produced malignancies in immunodeficient mice. Furthermore, we analyzed genomic instability in HiPSCs using whole-genome copy number variation analysis and determined that the extent of genomic instability was related with both the cells' propensity to form colonies and their potential for tumorigenesis. These findings indicate a risk for potential malignancy of HiPSCs derived from genomic instability and suggest that quality control tests, including comprehensive tumorigenicity assays and genomic integrity validation, should be rigorously executed before the clinical application of HiPSCs. In addition, HiPSCs should be generated through the use of combined factors or other approaches that decrease the likelihood of genomic instability.
关键词	Autophagy fusion oncoprotein acute myeloid leukemia
学科领域	Oncology
语种	英语
WOS研究方向	Oncology
来源期刊	CHINESE JOURNAL OF CANCER
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/111705
作者单位	Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol South China, Guangzhou 510060, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Liang, Yi,Zhang, Hui,Feng, Qi-Sheng,et al. The propensity for tumorigenesis in human induced pluripotent stem cells is related with genomic instability[J]. 中国科学院西北生态环境资源研究院,2013,32(4).
APA	Liang, Yi.,Zhang, Hui.,Feng, Qi-Sheng.,Cai, Man-Bo.,Deng, Wen.,...&Zeng, Yi-Xin.(2013).The propensity for tumorigenesis in human induced pluripotent stem cells is related with genomic instability.CHINESE JOURNAL OF CANCER,32(4).
MLA	Liang, Yi,et al."The propensity for tumorigenesis in human induced pluripotent stem cells is related with genomic instability".CHINESE JOURNAL OF CANCER 32.4(2013).