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DOI	10.1016/j.jinsphys.2011.03.026
	Cloning and expression analysis of six small heat shock protein genes in the common cutworm, Spodoptera litura
	Shen, Ying; Gu, Jun; Huang, Li-Hua; Zheng, Si-Chun; Liu, Lin; Xu, Wei-Hua; Feng, Qi-Li; Kang, Le
发表日期	2011
ISSN	0022-1910
卷号	57 期号:7
英文摘要	Small heat shock proteins (sHsps) are probably the most diverse in structure and function among the various superfamilies of stress proteins. To explore the diverse functions of insect sHsps, six sHsp cDNAs were cloned from the midgut cDNA library of Spodoptera litura, and a phylogenetic tree was constructed based on the conserved a-crystalline domains. The expression patterns in different developmental stages and tissues, as well as in response to both thermal and 20-hydroxyecdysone (20E) induction, were studied by real-time quantitative PCR. Based on sequence characteristics and phylogenetic relationships, the six SIHsps were classified into three independent groups: BmHsp20.4 like proteins (SIHsp19.7, 20.4, 20.7, 20.8), BmHsp26.6 like protein (SIHsp20), and BmHsp21.4 like protein (SIHsp21.4). All the SIHsps showed highest expression in the Malpighian tubules. The four BmHsp20.4 like protein genes were up-regulated by thermal stress and showed expression variation with development. SIHsp20 exhibited lower expression levels in both egg and larval stages than in pupal and adult stages. SIHsp21.4 retained a constant expression level during all life stages. The expression of both SIHsp20.4 and SIHsp20.8 was significantly up-regulated by 20E. These results indicate that sHsps play diverse functions in S. litura: the BmHsp20.4 like proteins are involved in both thermal adaptation and development: SIHsp20 does not respond to temperature stress but possibly plays a role in metamorphosis: SIHsp21.4 may have no direct relationship with either thermal response or development. (C) 2011 Elsevier Ltd. All rights reserved.
关键词	Small heat shock protein Thermal stress Metamorphosis 20-Hydroxyecdysone Real-time quantitative PCR Spodoptera litura
学科领域	Entomology; Physiology; Zoology
语种	英语
WOS研究方向	Entomology ; Physiology ; Zoology
来源期刊	JOURNAL OF INSECT PHYSIOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/111635
作者单位	S China Normal Univ, Sch Life Sci, Guangdong Prov Key Lab Biotechnol Plant Dev, Guangzhou 510631, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Shen, Ying,Gu, Jun,Huang, Li-Hua,et al. Cloning and expression analysis of six small heat shock protein genes in the common cutworm, Spodoptera litura[J]. 中国科学院西北生态环境资源研究院,2011,57(7).
APA	Shen, Ying.,Gu, Jun.,Huang, Li-Hua.,Zheng, Si-Chun.,Liu, Lin.,...&Kang, Le.(2011).Cloning and expression analysis of six small heat shock protein genes in the common cutworm, Spodoptera litura.JOURNAL OF INSECT PHYSIOLOGY,57(7).
MLA	Shen, Ying,et al."Cloning and expression analysis of six small heat shock protein genes in the common cutworm, Spodoptera litura".JOURNAL OF INSECT PHYSIOLOGY 57.7(2011).