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Regeneration of green plants from seed-derived callus cultures of Poa
Zong, Li; Ding, Lu-Ming; Xue, Xian; Wang, Tao
发表日期2010
ISSN1684-5315
卷号9期号:21
英文摘要Plants were regenerated from callus derived from entire mature seeds of three Poa pratensis cultivars (Kentucky bluegrass, Fylking and Baron) and one Chinese native species, Poa sphendyLodes L. Kentucky bluegrass showed the highest regenerating capacity of the four cultivars. Addition of low concentration (0.2 mg/l) of (6-benzyladenine) 6-BA to Murashige and Skoog (MS) callus induction medium containing 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) strongly stimulated seeds inducing shoot-forming callus cultures in Kentucky bluegrass. High percentage of shoots was induced in Shenh and Hilde-brandi (SH) medium containing low concentration (0.1 mg/l) of 2,4-D. Roots were obtained in SH medium with 0.1 mg/l NAA (naphthalene-acetic acid).
关键词Bluegrasscallus cultureregeneration systemgene transformation
学科领域Biotechnology & Applied Microbiology
语种英语
WOS研究方向Biotechnology & Applied Microbiology
来源期刊AFRICAN JOURNAL OF BIOTECHNOLOGY
来源机构中国科学院西北生态环境资源研究院
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/111591
作者单位Chinese Acad Sci, Key Lab Desert & Desertificat, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714
Zong, Li,Ding, Lu-Ming,Xue, Xian,et al. Regeneration of green plants from seed-derived callus cultures of Poa[J]. 中国科学院西北生态环境资源研究院,2010,9(21).
APA Zong, Li,Ding, Lu-Ming,Xue, Xian,&Wang, Tao.(2010).Regeneration of green plants from seed-derived callus cultures of Poa.AFRICAN JOURNAL OF BIOTECHNOLOGY,9(21).
MLA Zong, Li,et al."Regeneration of green plants from seed-derived callus cultures of Poa".AFRICAN JOURNAL OF BIOTECHNOLOGY 9.21(2010).
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