气候变化领域集成服务门户(CCPortal): Host barriers to SARS-CoV-2 demonstrated by ferrets in a high-exposure domestic setting

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DOI	10.1073/PNAS.2025601118
	Host barriers to SARS-CoV-2 demonstrated by ferrets in a high-exposure domestic setting
	Sawatzki K.; Hill N.J.; Puryear W.B.; Foss A.D.; Stone J.J.; Runstadler J.A.
发表日期	2021
ISSN	00278424
卷号	118 期号:18
英文摘要	Ferrets (Mustela putorius furo) are mustelids of special relevance to laboratory studies of respiratory viruses and have been shown to be susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and onward transmission. Here, we report the results of a natural experiment where 29 ferrets in one home had prolonged, direct contact and constant environmental exposure to two humans with symptomatic disease, one of whom was confirmed positive for SARS-CoV-2. We observed no evidence of SARS-CoV-2 transmission from humans to ferrets based on viral and antibody assays. To better understand this discrepancy in experimental and natural infection in ferrets, we compared SARS-CoV-2 sequences from natural and experimental mustelid infections and identified two surface glycoprotein Spike (S) mutations associated with mustelids. While we found evidence that angiotensin-converting enzyme II provides a weak host barrier, one mutation only seen in ferrets is located in the novel S1/S2 cleavage site and is computationally predicted to decrease furin cleavage efficiency. These data support the idea that host factors interacting with the novel S1/S2 cleavage site may be a barrier in ferret SARS-CoV-2 susceptibility and that domestic ferrets are at low risk of natural infection from currently circulating SARS-CoV-2. We propose two mechanistically grounded hypotheses for mustelid host adaptation of SARS-CoV-2, with possible effects that require additional investigation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Coronavirus; Genetics; SARS-CoV-2; Transmission; Virology
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181170
作者单位	Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, United States
推荐引用方式 GB/T 7714	Sawatzki K.,Hill N.J.,Puryear W.B.,et al. Host barriers to SARS-CoV-2 demonstrated by ferrets in a high-exposure domestic setting[J],2021,118(18).
APA	Sawatzki K.,Hill N.J.,Puryear W.B.,Foss A.D.,Stone J.J.,&Runstadler J.A..(2021).Host barriers to SARS-CoV-2 demonstrated by ferrets in a high-exposure domestic setting.Proceedings of the National Academy of Sciences of the United States of America,118(18).
MLA	Sawatzki K.,et al."Host barriers to SARS-CoV-2 demonstrated by ferrets in a high-exposure domestic setting".Proceedings of the National Academy of Sciences of the United States of America 118.18(2021).